Protonation States of the Tryptophan Synthase Internal Aldimine Active Site from Solid-State NMR Spectroscopy: Direct Observation of the Protonated Schiff Base Linkage to Pyridoxal-5′-Phosphate

[Image: see text] The acid–base chemistry that drives catalysis in pyridoxal-5′-phosphate (PLP)-dependent enzymes has been the subject of intense interest and investigation since the initial identification of PLP’s role as a coenzyme in this extensive class of enzymes. It was first proposed over 50...

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Main Authors: Caulkins, Bethany G., Bastin, Baback, Yang, Chen, Neubauer, Thomas J., Young, Robert P., Hilario, Eduardo, Huang, Yu-ming M., Chang, Chia-en A., Fan, Li, Dunn, Michael F., Marsella, Michael J., Mueller, Leonard J.
格式: Artigo
語言:Inglês
出版: American Chemical Society 2014
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4183654/
https://ncbi.nlm.nih.gov/pubmed/25148001
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja506267d
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