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A Novel In Vitro Assay to Assess Phosphorylation of 3′-[(18)F]fluoro-3′-Deoxythymidine

PURPOSE: 3′-[(18)F]fluoro-3’-deoxythymidine ([(18)F]FLT) is phosphorylated by thymidine kinase 1 (TK-1), a cell cycle regulated enzyme. Appropriate use of [(18)F]FLT tracer requires validation of the TK-1 activity. Here, we report development of a novel phosphoryl-transfer assay to assess phosphoryl...

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Bibliografiske detaljer
Main Authors: Guo, Ning, Xie, Jingping, Manning, H. Charles, Deane, Natasha G., Ansari, M. Sib, Coffey, Robert J., Gore, John, Price, Ronald R., Baldwin, Ronald M., McIntyre, J. Oliver
Format: Artigo
Sprog:Inglês
Udgivet: 2011
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4180216/
https://ncbi.nlm.nih.gov/pubmed/20532643
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s11307-010-0351-8
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