Capture and Amplification by Tailing and Switching (CATS): An ultrasensitive ligation-independent method for generation of DNA libraries for deep sequencing from picogram amounts of DNA and RNA

Massive parallel sequencing (MPS) technologies have paved the way into new areas of research including individualized medicine. However, sequencing of trace amounts of DNA or RNA still remains a major challenge, especially for degraded nucleic acids like circulating DNA. This together with high cost...

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Hlavní autoři: Turchinovich, Andrey, Surowy, Harald, Serva, Andrius, Zapatka, Marc, Lichter, Peter, Burwinkel, Barbara
Médium: Artigo
Jazyk:Inglês
Vydáno: Landes Bioscience 2014
Témata:
Technical Paper
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4179956/
https://ncbi.nlm.nih.gov/pubmed/24922482
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/rna.29304
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