Capture and Amplification by Tailing and Switching (CATS): An ultrasensitive ligation-independent method for generation of DNA libraries for deep sequencing from picogram amounts of DNA and RNA

Massive parallel sequencing (MPS) technologies have paved the way into new areas of research including individualized medicine. However, sequencing of trace amounts of DNA or RNA still remains a major challenge, especially for degraded nucleic acids like circulating DNA. This together with high cost...

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Bibliographic Details
Main Authors: Turchinovich, Andrey, Surowy, Harald, Serva, Andrius, Zapatka, Marc, Lichter, Peter, Burwinkel, Barbara
Format: Artigo
Language:Inglês
Published: Landes Bioscience 2014
Subjects:
Technical Paper
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4179956/
https://ncbi.nlm.nih.gov/pubmed/24922482
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/rna.29304
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