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Capture and Amplification by Tailing and Switching (CATS): An ultrasensitive ligation-independent method for generation of DNA libraries for deep sequencing from picogram amounts of DNA and RNA

Massive parallel sequencing (MPS) technologies have paved the way into new areas of research including individualized medicine. However, sequencing of trace amounts of DNA or RNA still remains a major challenge, especially for degraded nucleic acids like circulating DNA. This together with high cost...

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Main Authors: Turchinovich, Andrey, Surowy, Harald, Serva, Andrius, Zapatka, Marc, Lichter, Peter, Burwinkel, Barbara
Format: Artigo
Jezik:Inglês
Izdano: Landes Bioscience 2014
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4179956/
https://ncbi.nlm.nih.gov/pubmed/24922482
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/rna.29304
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