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Directed Evolution of Brain-Derived Neurotrophic Factor for Improved Folding and Expression in Saccharomyces cerevisiae

Brain-derived neurotrophic factor (BDNF) plays an important role in nervous system function and has therapeutic potential. Microbial production of BDNF has resulted in a low-fidelity protein product, often in the form of large, insoluble aggregates incapable of binding to cognate TrkB or p75 recepto...

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Autors principals: Burns, Michael L., Malott, Thomas M., Metcalf, Kevin J., Hackel, Benjamin J., Chan, Jonah R., Shusta, Eric V.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2014
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4178591/
https://ncbi.nlm.nih.gov/pubmed/25015885
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01466-14
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