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Direct elicitation of template concentration from quantification cycle (C(q)) distributions in digital PCR
Digital PCR (dPCR) exploits limiting dilution of a template into an array of PCR reactions. From this array the number of reactions that contain at least one (as opposed to zero) initial template is determined, allowing inferring the original template concentration. Here we present a novel protocol...
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| Main Authors: | , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
2014
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4176345/ https://ncbi.nlm.nih.gov/pubmed/25104023 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gku603 |
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