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Direct elicitation of template concentration from quantification cycle (C(q)) distributions in digital PCR

Digital PCR (dPCR) exploits limiting dilution of a template into an array of PCR reactions. From this array the number of reactions that contain at least one (as opposed to zero) initial template is determined, allowing inferring the original template concentration. Here we present a novel protocol...

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Bibliografski detalji
Glavni autori: Mojtahedi, Mitra, Fouquier d'Hérouël, Aymeric, Huang, Sui
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2014
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4176345/
https://ncbi.nlm.nih.gov/pubmed/25104023
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gku603
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