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Direct elicitation of template concentration from quantification cycle (C(q)) distributions in digital PCR

Digital PCR (dPCR) exploits limiting dilution of a template into an array of PCR reactions. From this array the number of reactions that contain at least one (as opposed to zero) initial template is determined, allowing inferring the original template concentration. Here we present a novel protocol...

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Detalhes bibliográficos
Main Authors: Mojtahedi, Mitra, Fouquier d'Hérouël, Aymeric, Huang, Sui
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4176345/
https://ncbi.nlm.nih.gov/pubmed/25104023
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gku603
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