One-step Purification of Twin-Strep-tagged Proteins and Their Complexes on Strep-Tactin Resin Cross-linked With Bis(sulfosuccinimidyl) Suberate (BS3)

Affinity purification of Strep-tagged fusion proteins on resins carrying an engineered streptavidin (Strep-Tactin) has become a widely used method for isolation of protein complexes under physiological conditions. Fusion proteins containing two copies of Strep-tag II, designated twin-Strep-tag or SI...

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Main Authors: Ivanov, Konstantin I, Bašić, Marta, Varjosalo, Markku, Mäkinen, Kristiina
Formáid: Artigo
Teanga:Inglês
Foilsithe: MyJove Corporation 2014
Ábhair:
Biochemistry
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4174720/
https://ncbi.nlm.nih.gov/pubmed/24796313
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51536
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