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Transcriptionally Profiling Cells Sorted by Transcript Abundance

We have developed a quantitative technique for sorting cells based on endogenous RNA abundance with a molecular resolution of 10-20 transcripts. We demonstrate efficient and unbiased RNA extraction from transcriptionally sorted cells and report a high fidelity transcriptome measurement of (mouse) in...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Klemm, Sandy, Semrau, Stefan, Wiebrands, Kay, Mooijman, Dylan, Faddah, Dina A., Jaenisch, Rudolf, van Oudenaarden, Alexander
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2014
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4174458/
https://ncbi.nlm.nih.gov/pubmed/24681693
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2910
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