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Increasing the reaction rate of hydroxynitrile lyase from Hevea brasiliensis towards mandelonitrile by copying active site residues from an esterase that accepts aromatic esters
The natural substrate of hydroxynitrile lyase from rubber tree (HbHNL, Hevea brasiliensis) is acetone cyanohydrin, but synthetic applications usually involve aromatic cyanohydrins such as mandelonitrile. To increase the activity of HbHNL toward this unnatural substrate, we replaced active site resid...
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| Hlavní autoři: | , , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
2014
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4167783/ https://ncbi.nlm.nih.gov/pubmed/25044660 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201402081 |
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