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Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting
Two-dimensional gel electrophoresis (2DE) is a powerful tool to uncover proteome modifications potentially related to different physiological or pathological conditions. Basically, this technique is based on the separation of proteins according to their isoelectric point in a first step, and secondl...
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Auteurs principaux: | , , , , , , , , , , , , , , , |
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Format: | Artigo |
Langue: | Inglês |
Publié: |
MyJove Corporation
2014
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Sujets: | |
Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4166172/ https://ncbi.nlm.nih.gov/pubmed/24747743 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51339 |
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