Consensus Brain-derived Protein, Extraction Protocol for the Study of Human and Murine Brain Proteome Using Both 2D-DIGE and Mini 2DE Immunoblotting

Two-dimensional gel electrophoresis (2DE) is a powerful tool to uncover proteome modifications potentially related to different physiological or pathological conditions. Basically, this technique is based on the separation of proteins according to their isoelectric point in a first step, and secondl...

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Detalhes bibliográficos
Main Authors: Fernandez-Gomez, Francisco-Jose, Jumeau, Fanny, Derisbourg, Maxime, Burnouf, Sylvie, Tran, Hélène, Eddarkaoui, Sabiha, Obriot, Hélène, Dutoit-Lefevre, Virginie, Deramecourt, Vincent, Mitchell, Valérie, Lefranc, Didier, Hamdane, Malika, Blum, David, Buée, Luc, Buée-Scherrer, Valérie, Sergeant, Nicolas
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2014
Assuntos:
Neuroscience
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4166172/
https://ncbi.nlm.nih.gov/pubmed/24747743
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51339
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