Biased estimates of clonal evolution and subclonal heterogeneity can arise from PCR duplicates in deep sequencing experiments

Accurate allele frequencies are important for measuring subclonal heterogeneity and clonal evolution. Deep-targeted sequencing data can contain PCR duplicates, inflating perceived read depth. Here we adapted the Illumina TruSeq Custom Amplicon kit to include single molecule tagging (SMT) and show th...

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Autors principals: Smith, Erin N, Jepsen, Kristen, Khosroheidari, Mahdieh, Rassenti, Laura Z, D’Antonio, Matteo, Ghia, Emanuela M, Carson, Dennis A, Jamieson, Catriona HM, Kipps, Thomas J, Frazer, Kelly A
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2014
Matèries:
Method
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4165357/
https://ncbi.nlm.nih.gov/pubmed/25103687
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13059-014-0420-4
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