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Effective strategy to assign (1)H-(15)N heteronuclear correlation NMR signals from lysine side-chain NH(3)(+) groups of proteins at low temperature
Recent studies have shown that lysine side-chain NH(3)(+) groups are excellent probes for NMR investigations of dynamics involving hydrogen bonds and ion pairs relevant to protein function. However, due to rapid hydrogen exchange, observation of (1)H-(15)N NMR cross peaks from lysine NH(3)(+) groups...
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| Main Authors: | , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
2014
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4160661/ https://ncbi.nlm.nih.gov/pubmed/25129623 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-014-9854-y |
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