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Effective strategy to assign (1)H-(15)N heteronuclear correlation NMR signals from lysine side-chain NH(3)(+) groups of proteins at low temperature

Recent studies have shown that lysine side-chain NH(3)(+) groups are excellent probes for NMR investigations of dynamics involving hydrogen bonds and ion pairs relevant to protein function. However, due to rapid hydrogen exchange, observation of (1)H-(15)N NMR cross peaks from lysine NH(3)(+) groups...

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Main Authors: Esadze, Alexandre, Zandarashvili, Levani, Iwahara, Junji
Formáid: Artigo
Teanga:Inglês
Foilsithe: 2014
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4160661/
https://ncbi.nlm.nih.gov/pubmed/25129623
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-014-9854-y
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