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In Vivo Incorporation of Unnatural Amino Acids Using the Chemical Aminoacylation Strategy. A Broadly Applicable Mechanistic Tool

We describe one strategy for incorporating unnatural amino acids site-specifically into proteins expressed in living cells, involving organic synthesis to chemically aminoacylate a suppressor tRNA, protein expression in Xenopus oocytes, and, primarily, monitoring protein function by electrophysiolog...

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Detalhes bibliográficos
Main Authors: Dougherty, Dennis A., Van Arnam, Ethan B.
Formato: Artigo
Idioma:Inglês
Publicado em: 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4155927/
https://ncbi.nlm.nih.gov/pubmed/24990307
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.201402080
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