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Improved amber and opal suppressor tRNAs for incorporation of unnatural amino acids in vivo. Part 1: Minimizing misacylation

The incorporation of unnatural amino acids site-specifically is a valuable technique for structure–function studies, incorporation of biophysical probes, and determining protein–protein interactions. THG73 is an amber suppressor tRNA used extensively for the incorporation of >100 different residu...

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Bibliografiske detaljer
Main Authors: Rodriguez, Erik A., Lester, Henry A., Dougherty, Dennis A.
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory Press 2007
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC1986802/
https://ncbi.nlm.nih.gov/pubmed/17698638
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.666807
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