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Evolution of Amber Suppressor tRNAs for Efficient Bacterial Production of Unnatural Amino Acid-Containing Proteins
[Image: see text] Regions of the M. jannaschii tyrosyl tRNA(CUA) thought to interact with elongation factor Tu were randomized, and the resulting tRNA libraries were subjected to in vitro evolution. The tRNAs identified resulted in significantly improved unnatural amino acid-containing protein yield...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2887739/ https://ncbi.nlm.nih.gov/pubmed/19856359 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.200904035 |
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