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Evolution of Amber Suppressor tRNAs for Efficient Bacterial Production of Unnatural Amino Acid-Containing Proteins

[Image: see text] Regions of the M. jannaschii tyrosyl tRNA(CUA) thought to interact with elongation factor Tu were randomized, and the resulting tRNA libraries were subjected to in vitro evolution. The tRNAs identified resulted in significantly improved unnatural amino acid-containing protein yield...

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Autors principals: Guo, Jiantao, Melançon, Charles E., Lee, Hyun Soo, Groff, Dan, Schultz, Peter G.
Format: Artigo
Idioma:Inglês
Publicat: 2009
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2887739/
https://ncbi.nlm.nih.gov/pubmed/19856359
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.200904035
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