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In vitro incorporation of nonnatural amino acids into protein using tRNA(Cys)-derived opal, ochre, and amber suppressor tRNAs

Amber suppressor tRNAs are widely used to incorporate nonnatural amino acids into proteins to serve as probes of structure, environment, and function. The utility of this approach would be greatly enhanced if multiple probes could be simultaneously incorporated at different locations in the same pro...

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Detalhes bibliográficos
Main Authors: Gubbens, Jacob, Kim, Soo Jung, Yang, Zhongying, Johnson, Arthur E., Skach, William R.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2905763/
https://ncbi.nlm.nih.gov/pubmed/20581130
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.2024810
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