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Harnessing CRISPR-Cas9 immunity for genetic engineering
CRISPR-Cas encodes an adaptive immune system that defends prokaryotes against infectious viruses and plasmids. Immunity is mediated by Cas nucleases, which use small RNA guides (the crRNAs) to specify a cleavage site within the genome of invading nucleic acids. In type II CRISPR-Cas systems, the DNA...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2014
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4155128/ https://ncbi.nlm.nih.gov/pubmed/25048165 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mib.2014.07.001 |
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