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Optimization of Genome Engineering Approaches with the CRISPR/Cas9 System
Designer nucleases such as TALENS and Cas9 have opened new opportunities to scarlessly edit the mammalian genome. Here we explored several parameters that influence Cas9-mediated scarless genome editing efficiency in murine embryonic stem cells. Optimization of transfection conditions and enriching...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Public Library of Science
2014
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4148324/ https://ncbi.nlm.nih.gov/pubmed/25166277 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0105779 |
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