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Self-processing of a barley subtilase expressed in E. coli

The barley protease BAJ93208 belongs to the subtilase family of serine proteases. We have expressed BAJ93208 in the cytoplasm of the Escherichiacoli strain SHuffle C3030 using a rhamnose-inducible promoter. The expression construct included a (His)(6)-tag at the N-terminus and a strep-tag at the C-t...

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Bibliografiske detaljer
Main Authors: Plattner, Stephan, Gruber, Clemens, Altmann, Friedrich, Bohlmann, Holger
Format: Artigo
Sprog:Inglês
Udgivet: Academic Press 2014
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4148201/
https://ncbi.nlm.nih.gov/pubmed/24927642
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2014.05.014
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