Conditional site-specific recombination in mammalian cells using a ligand-dependent chimeric Cre recombinase.
We have developed a strategy to generate mutant genes in mammalian cells in a conditional manner by employing a fusion protein, Cre-ER, consisting of the loxP site-specific Cre recombinase linked to the ligand-binding domain of the human estrogen receptor. We have established homozygous retinoid X r...
Uloženo v:
| Vydáno v: | Proc Natl Acad Sci U S A |
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| Hlavní autoři: | , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
National Academy of Sciences
1995
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC41457/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7624356/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.92.15.6991 |
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