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Detecting Kinase Activities from Single Cell Lysate Using Concentration-Enhanced Mobility Shift Assay

[Image: see text] Electrokinetic preconcentration coupled with mobility shift assays can give rise to very high detection sensitivities. We describe a microfluidic device that utilizes this principle to detect cellular kinase activities by simultaneously concentrating and separating substrate peptid...

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Detalhes bibliográficos
Main Authors: Cheow, Lih Feng, Sarkar, Aniruddh, Kolitz, Sarah, Lauffenburger, Douglas, Han, Jongyoon
Formato: Artigo
Idioma:Inglês
Publicado em: American Chemical Society 2014
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4144746/
https://ncbi.nlm.nih.gov/pubmed/25025773
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac502185v
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