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Enhancing Protease Activity Assay in Droplet-Based Microfluidics Using a Biomolecule Concentrator

We introduce an integrated microfluidic device consisting of a biomolecule concentrator and a microdroplet generator, which enhances the limited sensitivity of low-abundance enzyme assays by concentrating biomolecules before encapsulating them into droplet microreactors. We used this platform to det...

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Detalhes bibliográficos
Main Authors: Chen, Chia-Hung, Sarkar, Aniruddh, Song, Yong-Ak, Miller, Miles A., Kim, Sung Jae, Griffith, Linda G., Lauffenburger, Douglas A., Han, Jongyoon
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3165005/
https://ncbi.nlm.nih.gov/pubmed/21671557
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja2036628
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