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Effective Protein Separation by Coupling Hydrophobic Interaction and Reverse Phase Chromatography for Top-down Proteomics

[Image: see text] One of the challenges in proteomics is the proteome’s complexity, which necessitates the fractionation of proteins prior to the mass spectrometry (MS) analysis. Despite recent advances in top-down proteomics, separation of intact proteins remains challenging. Hydrophobic interactio...

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Main Authors: Xiu, Lichen, Valeja, Santosh G., Alpert, Andrew J., Jin, Song, Ge, Ying
格式: Artigo
語言:Inglês
出版: American Chemical Society 2014
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4144745/
https://ncbi.nlm.nih.gov/pubmed/24968279
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac501836k
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