Temperature and Electrolyte Optimization of the α-Hemolysin Latch Sensing Zone for Detection of Base Modification in Double-Stranded DNA

The latch region of the wild-type protein pore α-hemolysin (α-HL) constitutes a sensing zone for individual abasic sites (and furan analogs) in double-stranded DNA (dsDNA). The presence of an abasic site or furan within a DNA duplex, electrophoretically captured in the α-HL vestibule and positioned...

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Detalhes bibliográficos
Main Authors: Johnson, Robert P., Fleming, Aaron M., Jin, Qian, Burrows, Cynthia J., White, Henry S.
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2014
Assuntos:
Membranes
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4142235/
https://ncbi.nlm.nih.gov/pubmed/25140427
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2014.07.006
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