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Colloidal lenses allow high-temperature single-molecule imaging and improve fluorophore photostability
Although single-molecule fluorescence spectroscopy was first demonstrated at near-absolute zero temperatures (1.8 K)(1), the field has since advanced to include room-temperature observations(2), largely owing to the use of objective lenses with high numerical aperture, brighter fluorophores and more...
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| Auteurs principaux: | , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2009
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4141882/ https://ncbi.nlm.nih.gov/pubmed/20023643 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nnano.2009.452 |
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