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Conditional targeting of Ispd using paired Cas9 nickase and a single DNA template in mice
CRISPR/Cas9 technology is a highly promising genome editing tool in the mouse, potentially overcoming the costs and time required for more traditional gene targeting methods in embryonic stem (ES) cells. Recently, compared to the wildtype nuclease, paired Cas9 nickase (Cas9n) combined with single gu...
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| Hlavní autoři: | , |
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| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Elsevier
2014
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4141200/ https://ncbi.nlm.nih.gov/pubmed/25161872 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.fob.2014.06.007 |
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