In vitro genetic analysis of the structural features of the pre-tRNA required for determination of the 3' splice site in the intron excision reaction.

During processing of intron-containing pre-tRNAs, the Xenopus laevis splicing endonuclease binds the precursor and cleaves it at both the 5' and 3' splice sites. In vitro selection was used to determine structural features characteristic of precursor tRNA molecules that are active in this...

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Bibliographic Details
Main Authors: Bufardeci, E, Fabbri, S, Baldi, M I, Mattoccia, E, Tocchini-Valentini, G P
Format: Artigo
Language:Inglês
Published: 1993
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC413913/
https://ncbi.nlm.nih.gov/pubmed/8223479
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