Non-enzymatic excision of pre-tRNA introns?
We used human tRNA(Tyr) precursor as a substrate to study self-excision of a pre-tRNA intron. This RNA was synthesized in vitro in a HeLa cell extract. It contains a 5' leader, an intron of 20 nucleotides and a 3' trailer. Self-cleavage of pre-tRNA(Tyr) occurs in 100 mM NH4OAc at a pH ranging from 6...
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| Publicado no: | EMBO J |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group
1989
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC400802/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2714254/ https://ncbi.nlm.nih.govhttps://doi.org/10.1002/j.1460-2075.1989.tb03376.x |
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