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Quantification of gene transcripts with Deep Sequencing Analysis of Gene Expression (DSAGE) from 1-2μg total RNA
Deep sequencing analysis of gene expression (DSAGE) measures global gene transcript levels by massively parallel sequencing of cDNA tags, using 1-2μg total RNA. 21-bp cDNA tags are generated by NlaIII digestion of the cDNA, followed by MmeI cleavage offset from the NlaIII site. cDNA tags are then qu...
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| Hauptverfasser: | , , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2011
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4139004/ https://ncbi.nlm.nih.gov/pubmed/21225638 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471142727.mb25b09s93 |
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