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Quantification of gene transcripts with Deep Sequencing Analysis of Gene Expression (DSAGE) from 1-2μg total RNA

Deep sequencing analysis of gene expression (DSAGE) measures global gene transcript levels by massively parallel sequencing of cDNA tags, using 1-2μg total RNA. 21-bp cDNA tags are generated by NlaIII digestion of the cDNA, followed by MmeI cleavage offset from the NlaIII site. cDNA tags are then qu...

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Detalhes bibliográficos
Main Authors: Christodoulou, DC, Gorham, JM, Kawana, M, DePalma, SR, Herman, DS, Wakimoto, H
Formato: Artigo
Idioma:Inglês
Publicado em: 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4139004/
https://ncbi.nlm.nih.gov/pubmed/21225638
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471142727.mb25b09s93
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