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Setting objective thresholds for rare event detection in flow cytometry
The accurate identification of rare antigen-specific cytokine positive cells from peripheral blood mononuclear cells (PBMC) after antigenic stimulation in an intracellular staining (ICS) flow cytometry assay is challenging, as cytokine positive events may be fairly diffusely distributed and lack an...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4138273/ https://ncbi.nlm.nih.gov/pubmed/24727143 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2014.04.002 |
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