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Setting objective thresholds for rare event detection in flow cytometry

The accurate identification of rare antigen-specific cytokine positive cells from peripheral blood mononuclear cells (PBMC) after antigenic stimulation in an intracellular staining (ICS) flow cytometry assay is challenging, as cytokine positive events may be fairly diffusely distributed and lack an...

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Autori principali: Richards, Adam J., Staats, Janet, Enzor, Jennifer, McKinnon, Katherine, Frelinger, Jacob, Denny, Thomas N., Weinhold, Kent J., Chan, Cliburn
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2014
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4138273/
https://ncbi.nlm.nih.gov/pubmed/24727143
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2014.04.002
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