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Setting objective thresholds for rare event detection in flow cytometry

The accurate identification of rare antigen-specific cytokine positive cells from peripheral blood mononuclear cells (PBMC) after antigenic stimulation in an intracellular staining (ICS) flow cytometry assay is challenging, as cytokine positive events may be fairly diffusely distributed and lack an...

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Détails bibliographiques
Auteurs principaux: Richards, Adam J., Staats, Janet, Enzor, Jennifer, McKinnon, Katherine, Frelinger, Jacob, Denny, Thomas N., Weinhold, Kent J., Chan, Cliburn
Format: Artigo
Langue:Inglês
Publié: 2014
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4138273/
https://ncbi.nlm.nih.gov/pubmed/24727143
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2014.04.002
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