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The Role of Mass Transport Limitation and Surface Heterogeneity in the Biophysical Characterization of Macromolecular Binding Processes by SPR Biosensing

This chapter presents an introduction to the kinetic analysis of SPR biosensor data for the determination of affinity and kinetic rate constants of biomolecular interactions between an immobilized and a soluble binding partner. The need to be aware of and critically tests the assumptions underlying...

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Detalles Bibliográficos
Main Authors: Schuck, Peter, Zhao, Huaying
Formato: Artigo
Idioma:Inglês
Publicado: 2010
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4134667/
https://ncbi.nlm.nih.gov/pubmed/20217612
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60761-670-2_2
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