Random and targeted transgene insertion in C. elegans using a modified Mosl transposon

We have generated a recombinant Mos1 transposon that can insert up to 45 kb transgenes into the C. elegans genome. The minimal Mos1 transposon (miniMos) is 550 bp long and inserts DNA into the genome at high frequency (~60% of injected animals). Genetic and antibiotic markers can be used for selecti...

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Hlavní autoři: Frokjaer-Jensen, Christian, Davis, M Wayne, Sarov, Mihail, Taylor, Jon, Flibotte, Stephane, LaBella, Matthew, Pozniakovski, Andrei, Moerman, Donald G, Jorgensen, Erik M
Médium: Artigo
Jazyk:Inglês
Vydáno: 2014
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4126194/
https://ncbi.nlm.nih.gov/pubmed/24820376
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2889
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