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Random and targeted transgene insertion in C. elegans using a modified Mosl transposon
We have generated a recombinant Mos1 transposon that can insert up to 45 kb transgenes into the C. elegans genome. The minimal Mos1 transposon (miniMos) is 550 bp long and inserts DNA into the genome at high frequency (~60% of injected animals). Genetic and antibiotic markers can be used for selecti...
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| Main Authors: | , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4126194/ https://ncbi.nlm.nih.gov/pubmed/24820376 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2889 |
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