High-level expression of functional rat neuronal nitric oxide synthase in Escherichia coli.

The neuronal nitric oxide synthase (nNOS) has been successfully overexpressed in Escherichia coli, with average yields of 125-150 nmol (20-24 mg) of enzyme per liter of cells. The cDNA for nNOS was subcloned into the pCW vector under the control of the tac promotor and was coexpressed with the chape...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Roman, L J, Sheta, E A, Martasek, P, Gross, S S, Liu, Q, Masters, B S
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1995
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC41170/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7545302/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.92.18.8428
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