Quantitative continuous assay for hyaluronan synthase

A rapid, continuous, and convenient three-enzyme coupled UV absorption assay was developed to quantitate the glucuronic acid and N-acetylglucosamine transferase activities of hyaluronan synthase from Pasteurella multocida (PmHAS). Activity was measured by coupling the UDP produced from the PmHAS-cat...

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Bibliografski detalji
Glavni autori: Krupa, Joanne C., Shaya, David, Chi, Lianli, Linhardt, Robert J., Cygler, Miroslaw, Withers, Stephen G., Mort, John S.
Format: Artigo
Jezik:Inglês
Izdano: 2006
Teme:
Article
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4114249/
https://ncbi.nlm.nih.gov/pubmed/17173853
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2006.11.011
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