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Growth and maintenance of HeLa cells in serum-free medium supplemented with hormones.

HeLa cells grow in a nutritionally complete synthetic medium (Ham's F12) supplemented with insulin, transferrin, hydrocortisone (aldosterone), fibroblast growth factor, and epidermal growth factor. This hormone-supplemented medium supports clonal growth, long-term cultivation, and a growth rate equa...

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Bibliografski detalji
Izdano u:Proc Natl Acad Sci U S A
Glavni autori: Hutchings, S E, Sato, G H
Format: Artigo
Jezik:Inglês
Izdano: National Academy of Sciences 1978
Teme:
Online pristup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC411365/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/273251/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.75.2.901
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