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Growth and maintenance of HeLa cells in serum-free medium supplemented with hormones.
HeLa cells grow in a nutritionally complete synthetic medium (Ham's F12) supplemented with insulin, transferrin, hydrocortisone (aldosterone), fibroblast growth factor, and epidermal growth factor. This hormone-supplemented medium supports clonal growth, long-term cultivation, and a growth rate...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1978
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC411365/ https://ncbi.nlm.nih.gov/pubmed/273251 |
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