Growth and maintenance of HeLa cells in serum-free medium supplemented with hormones.
HeLa cells grow in a nutritionally complete synthetic medium (Ham's F12) supplemented with insulin, transferrin, hydrocortisone (aldosterone), fibroblast growth factor, and epidermal growth factor. This hormone-supplemented medium supports clonal growth, long-term cultivation, and a growth rate equa...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1978
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC411365/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/273251/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.75.2.901 |
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