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Single-mRNA counting using fluorescent in situ hybridization in budding yeast
Fluorescent in situ hybridization (FISH) allows the quantification of single mRNAs in budding yeast using fluorescently labeled single-stranded DNA probes, a wide-field epifluorescence microscope and a spot-detection algorithm. Fixed yeast cells are attached to coverslips and hybridized with a mixtu...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2012
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4112553/ https://ncbi.nlm.nih.gov/pubmed/22301778 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2011.451 |
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