Single-mRNA counting using fluorescent in situ hybridization in budding yeast

Fluorescent in situ hybridization (FISH) allows the quantification of single mRNAs in budding yeast using fluorescently labeled single-stranded DNA probes, a wide-field epifluorescence microscope and a spot-detection algorithm. Fixed yeast cells are attached to coverslips and hybridized with a mixtu...

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Autori principali: Trcek, Tatjana, Chao, Jeffrey A, Larson, Daniel R, Park, Hye Yoon, Zenklusen, Daniel, Shenoy, Shailesh M, Singer, Robert H
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2012
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4112553/
https://ncbi.nlm.nih.gov/pubmed/22301778
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2011.451
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