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Random mutagenesis by error-prone Pol I plasmid replication in Escherichia coli

Directed evolution is an approach that mimics natural evolution in the laboratory with the goal of modifying existing enzymatic activities or of generating new ones. The identification of mutants with desired properties involves the generation of genetic diversity coupled with a functional selection...

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Autori principali: Alexander, David L., Lilly, Joshua, Hernandez, Jaime, Romsdahl, Jillian, Troll, Christopher J., Camps, Manel
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2014
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4112372/
https://ncbi.nlm.nih.gov/pubmed/25055769
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-1053-3_3
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