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Random mutagenesis by error-prone Pol I plasmid replication in Escherichia coli

Directed evolution is an approach that mimics natural evolution in the laboratory with the goal of modifying existing enzymatic activities or of generating new ones. The identification of mutants with desired properties involves the generation of genetic diversity coupled with a functional selection...

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Hlavní autoři: Alexander, David L., Lilly, Joshua, Hernandez, Jaime, Romsdahl, Jillian, Troll, Christopher J., Camps, Manel
Médium: Artigo
Jazyk:Inglês
Vydáno: 2014
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4112372/
https://ncbi.nlm.nih.gov/pubmed/25055769
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-1053-3_3
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