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Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity
Cytolytic activity of CD8+ T cells is rarely evaluated. We describe here a new cell-based assay to measure the capacity of antigen-specific CD8+ T cells to kill CD4+ T cells loaded with their cognate peptide. Target CD4+ T cells are divided into two populations, labeled with two different concentrat...
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| Главные авторы: | , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
MyJove Corporation
2013
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4111270/ https://ncbi.nlm.nih.gov/pubmed/24378436 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51105 |
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