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Cell-based Flow Cytometry Assay to Measure Cytotoxic Activity

Cytolytic activity of CD8+ T cells is rarely evaluated. We describe here a new cell-based assay to measure the capacity of antigen-specific CD8+ T cells to kill CD4+ T cells loaded with their cognate peptide. Target CD4+ T cells are divided into two populations, labeled with two different concentrat...

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Détails bibliographiques
Auteurs principaux: Noto, Alessandra, Ngauv, Pearline, Trautmann, Lydie
Format: Artigo
Langue:Inglês
Publié: MyJove Corporation 2013
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4111270/
https://ncbi.nlm.nih.gov/pubmed/24378436
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51105
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