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Functional reconstitution of monomeric CYP3A4 with multiple cytochrome P450 reductase molecules in Nanodiscs

Traditional reconstitution of membrane cytochromes P450 monooxygenase system requires efficient solubilization of both P450 heme enzymes and redox partner NADPH dependent reductase, CPR, either in mixed micellar solution or by incorporation in liposomes. Here we describe a simple alternative approac...

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Bibliografische gegevens
Hoofdauteurs: Grinkova, Yelena V., Denisov, Ilia G., Sligar, Stephen G.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2010
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4103192/
https://ncbi.nlm.nih.gov/pubmed/20599740
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrc.2010.06.058
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