Preparation of Primary Neurons for Visualizing Neurites in a Frozen-hydrated State Using Cryo-Electron Tomography

Neurites, both dendrites and axons, are neuronal cellular processes that enable the conduction of electrical impulses between neurons. Defining the structure of neurites is critical to understanding how these processes move materials and signals that support synaptic communication. Electron microsco...

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Bibliographic Details
Main Authors: Shahmoradian, Sarah H., Galiano, Mauricio R., Wu, Chengbiao, Chen, Shurui, Rasband, Matthew N., Mobley, William C., Chiu, Wah
Format: Artigo
Language:Inglês
Published: MyJove Corporation 2014
Subjects:
Neuroscience
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4089403/
https://ncbi.nlm.nih.gov/pubmed/24561719
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/50783
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